CK-MB assay in the Ames Seralyzer.

نویسنده

  • R S Harding
چکیده

aminobutyric acid in amino acid position 2 of the molecule. In animnig CyG retains the immunosuppressive properties of CyA but is less nephrotoxic (Hiestand et al., Transplant Proc 1985;17:1362-4; Caine et al., Lancet 1985i:1342). Future studies in humans are planned but a method for quantifying CyG is required. We assessed a radioimmunoassay for CrG in plammi, using CyG standards, CyA antibody, and [ HJCyA tracer, the latter two available in a kit for CyA quantification supplied by Sandoz Inc., Basel, Switzerland. We found that the CyA antibody showed equal affinity for CyA and CyG. The standard curve obtained for CyG was almost identical to that obtained for CyA. The precision of the CyG assay was similar to that of the CyA assay, withinand between-run CVs being <15%. The sensitivity (detection limit) of the assay was 25 tg/L, and the mean analytical recovery of the drug was near 100%. We conclude that routine monitoring of CyG is possible with the currently available RIA method used for quantifying CyA.

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عنوان ژورنال:
  • Clinical chemistry

دوره 32 4  شماره 

صفحات  -

تاریخ انتشار 1986